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Illumina Miseq測序儀測序必需試劑盒,包括簇生成、雙端測序和邊合成邊測序試劑(SBS)。
Illumina- MiSeq Kit
產(chǎn)品英文名稱(chēng):MiSeq Kit
產(chǎn)品中文名稱(chēng):MiSeq Kit試劑盒
用途:小型全基因組測序(微生物、病毒),靶向基因測序(擴增子、基因panel),16S宏基因組測序,靶向基因表達譜分析,miRNA和Small RNA分析,長(cháng)片段擴增子測序,DNA-蛋白質(zhì)相互作用分析
貨號:
MS-102-2001/MS-102-2002/MS-102-2003/MS-102-3001/MS-102-3003/MS-103-1001/MS-103-1002/MS-103-1003
MiSeq Kit介紹
要在 MiSeq 上執行運行,需要一個(gè)一次性 MiSeq 試劑盒。
該試劑盒有各種不同的類(lèi)型和規格。每種類(lèi)型的 MiSeq 試劑盒都包含一個(gè)試劑盒的流動(dòng)槽類(lèi)型和執行一次運行所需的全部試劑。試劑盒中提供的流動(dòng)槽、PR2 瓶和試劑盒均使用射頻識別(radio-frequency identification,簡(jiǎn)稱(chēng) RFID)進(jìn)行精確的耗材跟蹤并確保兼容性。始終使用與您的流動(dòng)槽類(lèi)型相匹配的試劑盒。如果試劑盒不兼容,運行設置期間會(huì )顯示一條消息,提示您裝入兼容的試劑盒。
流動(dòng)槽:flow cell
MiSeq Kit特點(diǎn):
改良化學(xué)過(guò)程后,循環(huán)時(shí)間更快、讀長(cháng)(reads)更長(cháng)、輸出更多
雙表面成像實(shí)現的讀長(cháng)數是v1試劑盒單表面成像的兩倍
使用500次循環(huán)的試劑盒可擴展讀長(cháng)長(cháng)度
選擇您應用的循環(huán)次數(50、300或500)
借助MiSeq試劑盒v3,研究人員可:
使單次運行的輸出翻倍
使用600次循環(huán)試劑盒,使reads長(cháng)度zui長(cháng)延伸至2 x 300堿基對
將reads數量增至2500萬(wàn)個(gè),以開(kāi)創(chuàng )新的應用
The MiSeq Reagent Kit is a single-use reagent kit required to perform a sequencing run. It is available in different types and sizes. Each type of MiSeq Reagent Kit includes a kit-specific flow cell type and all reagents required for performing a run.The flow cell, PR2 bottle, and reagent cartridge provided in the kit use radio-frequency identification (RFID) for accurate consumable tracking and compatibility.Always use the reagent cartridge associated with your flow cell type. If the reagent cartridge is not compatible, a message appears during run setup that prompts you to load a compatible reagent cartridge.
Get faster cycle times, longer reads and more output from improved chemistry
Dual surface imaging enables twice the number of reads compared to single surface imaging with the v1 kit
Extend your read lengths with the 500-cycle kit
Choose the perfect number of cycles for your application (50, 300, or 500)
MiSeq Reagent Kit v3 allows researchers to:
Double the output of a single run
Extend read lengths up to 2 x 300 bp with the 600-cycle kit
Increase the number of reads to 25 million to unlock new applications
MiSeq Kit應用
小型全基因組測序(微生物、病毒),靶向基因測序(擴增子、基因panel),16S宏基因組測序,靶向基因表達譜分析,miRNA和Small RNA分析,長(cháng)片段擴增子測序,DNA-蛋白質(zhì)相互作用分析
實(shí)例:
上游試劑 癌癥panel:TruSeq Amplicon - Cancer Panel
Illumina MiSeq Kit 參數
zui高通量 | 8.5 Gb (500-cycle MiSeq Reagent Kit v2), 5.1 Gb (300-cycle MiSeq Reagent Kit v2), 1.2 Gb (300-cycle MiSeq Reagent Micro Kit v2), 0.850 Gb (50-cycle MiSeq Reagent Kit v2), 0.5 Gb (500-cycle MiSeq Reagent Nano Kit v2), 0.3 Gb (300-cycle MiSeq Reagent Nano Kit v2),15 Gb (600-cycle MiSeq Reagent Kit v3), 3.8 Gb (150-cycle MiSeq Reagent Kit v3) |
zui高讀長(cháng) | Up to 15 million(v2),Up to 25 Million(v3) |
試劑類(lèi)型 | Cluster Generation, Paired-End Sequencing, Sequencing by Synthesis |
測序儀 | MiSeq, MiSeq FGx in Research Mode, MiSeqDx in Research Mode |
組份 | 數量 | 描述 | 保存 |
Reagent Cartridge | 1 | Single-use pre-filled cartridge | -25°C to -15°C |
HT1 | 1 | 5 ml tube, Hybridization Buffer | -25°C to -15°C |
Flow Cell | 1 | Single-use flow cell | 2°C to 8°C |
PR2 | 1 | 500 ml bottle, Incorporation Buffer | 2°C to 8°C |
自備試劑:
備用的 1.0 N NaOH,分子生物學(xué)級
酒精擦拭紙,70%甘油或乙醇,70%
一次性無(wú)粉手套
低塵實(shí)驗室棉巾紙
微量離心管
擦鏡紙,4 x 6 英寸
MiSeq試管,Illumina
NaOCl,5%
Tween 20
實(shí)驗室級用水
訂購信息
品牌 | 貨號 | 名稱(chēng) | 規格 |
Illumina | MS-102-2001 | MiSeq Reagent Kit v2 | 50 cycles |
Illumina | MS-102-2002 | MiSeq Reagent Kit v2 | 300 cycles |
Illumina | MS-102-2003 | MiSeq Reagent Kit v2 | 500 cycles |
Illumina | MS-102-3001 | MiSeq Reagent Kit v3 | 150 cycles |
Illumina | MS-102-3003 | MiSeq Reagent Kit v3 | 600 cycles |
Illumina | MS-103-1001 | MiSeq Reagent Nano Kit v2 | 300 cycles |
Illumina | MS-103-1002 | MiSeq Reagent Micro Kit v2 | 300 cycles |
Illumina | MS-103-1003 | MiSeq Reagent Nano Kit v2 | 500 cycles |
注:micro 和 nano的區別在于測序通量。
Illumina- MiSeq Kit及更多測序產(chǎn)品詳詢(xún)
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